Journal: Biochemistry and Biophysics Reports

Article Title: 8-O-acetylharpagide, an active compound isolated from A. taiwanensis selectively induced G2/M phase arrest and radiosensitivity in hypopharyngeal cancer cells

doi: 10.1016/j.bbrep.2025.102431

Figure Lengend Snippet: Effects of AT-1 on FaDu cells and PDL cells. (A) Cell viability was assessed using the MTT assay after FaDu cells and PDL cells were treated with different concentrations of AT-1 for 24 h. (B) Images of FaDu and PDL cells treated with AT-1 (1 mM) or DMSO for 24 h. Red arrows indicate rounded, mitosis-like cells and yellow arrows indicate unchanged cells for demonstration. Scale bar = 100 μm. (C) Quantification of rounded cells from cell images. (D) Comparison of cell proliferative rates of FaDu cells treated with AT-1 or DMSO. (E) Quantification of intracellular ROS levels using the DCFDA fluorescence assay. Data are normalized to cell viability (mean ± SEM, n = 3). ∗ and #: p < 0.05, ∗∗ and ###: p < 0.005, ##: p < 0.01, ∗∗∗: p < 0.001, and ∗∗∗∗: p < 0.0001 compared to the control group.

Article Snippet: Human HPC FaDu cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA).

Techniques: MTT Assay, Comparison, Fluorescence, Control

Journal: Biochemistry and Biophysics Reports

Article Title: 8-O-acetylharpagide, an active compound isolated from A. taiwanensis selectively induced G2/M phase arrest and radiosensitivity in hypopharyngeal cancer cells

doi: 10.1016/j.bbrep.2025.102431

Figure Lengend Snippet: AT-1 induced G2/M phase arrest in FaDu cells but not PDL cells. (A) and (B) Flow cytometric analysis of cell cycle distribution in FaDu cells and PDL cells treated with or without AT-1 for 24 h, respectively. (C) Quantification of cell cycle phases (Sub G1, G0/G1, S, G2/M) in FaDu and PDL cells. Data were presented as mean ± SEM from three independent experiments. (D) Western blot analysis of cell cycles related proteins in FaDu cells and PDL cells treated with AT-1. Cyclin B1, CDK1, p21, and CHK1 protein expression levels were normalized to GAPDH. (E) Summary bar graphs for Western blot quantification (mean ± SEM, n = 3). ∗ and #: p < 0.05, ∗∗ and ###: p < 0.005 compared to the control group.

Article Snippet: Human HPC FaDu cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA).

Techniques: Western Blot, Expressing, Control

Journal: Biochemistry and Biophysics Reports

Article Title: 8-O-acetylharpagide, an active compound isolated from A. taiwanensis selectively induced G2/M phase arrest and radiosensitivity in hypopharyngeal cancer cells

doi: 10.1016/j.bbrep.2025.102431

Figure Lengend Snippet: Effects of AT-1 induced apoptosis in FaDu cells and PDL cells. (A) Flow cytometric profiles of annexin V/PI dual staining in FaDu cells and PDL cells with different treatments for 24 h. (B) and (C) Quantification of necrosis-like, late apoptosis, and early apoptosis in FaDu cells and PDL cells, respectively. (D) Western blot analysis of apoptotic related proteins in FaDu and PDL cells treated with AT-1. AKT, pAKT, and BCL-2 protein expression levels were normalized to GAPDH. (E) Summary bar graphs for Western blot quantification (mean ± SEM, n = 3) ∗: p < 0.05, ∗∗∗∗: p < 0.001.

Article Snippet: Human HPC FaDu cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA).

Techniques: Staining, Western Blot, Expressing

Journal: Biochemistry and Biophysics Reports

Article Title: 8-O-acetylharpagide, an active compound isolated from A. taiwanensis selectively induced G2/M phase arrest and radiosensitivity in hypopharyngeal cancer cells

doi: 10.1016/j.bbrep.2025.102431

Figure Lengend Snippet: Effects of AT-1 on radiosensitivity of FaDu cells and PDL cells. (A) and (B) MTT assay for analysis of the cell viability of FaDu cells and PDL cells irradiated with different doses of X-rays after AT-1 treatment, respectively. Cell viability was presented as fractions relative to the control group (0 Gy, set as 1). ∗∗∗∗: p < 0.0001.

Article Snippet: Human HPC FaDu cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and maintained in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA).

Techniques: MTT Assay, Irradiation, Control

Journal: bioRxiv

Article Title: IL-1α drives a tumor-stroma-neutrophil axis through inflammatory fibroblast activation in head and neck cancer

doi: 10.64898/2026.01.20.700440

Figure Lengend Snippet: (A) Kaplan-Meier analysis showing that neutrophil density negatively correlates with patient outcome, n=83 patients. (B) CD66b + TAN were analyzed by immunofluorescence and quantified in tumor, tumor margin and stroma of HNSCC patients (mean ± SD, n=83, circles = males, open circles = females). (C) Schematic overview of the in vitro system for tumor-stroma communication, generated using BioRender. (D) SNs from tumor (FaDu, PCI13), MSC-primed tumor, MSCs and tumor-primed MSCs were analysed for released CXCL8 by ELISA, mean + SD, n=3. (E) The impact of these SN on PMN chemotaxis was analyzed using transwell assays, mean + SD, n=3-8. (F) Luminex screening was performed on SNs from FaDu cells and seven patient-derived MSC lines, analyzed both in their naïve state and after FaDu-priming. CXCL8 was measured using ELISA. Data are depicted in pg/mL. Statistical analysis was performed with Kruskal-Wallis (B) and ordinary one-way ANOVA (D, E). P -values are indicated.

Article Snippet: The head and neck cancer cell lines FaDu (ATCC HTB-43) and PCI13 (kindly provided by the Pittsburgh Cancer Institute) were cultured in RPMI-1640 (Pan-Biotech), supplemented with 10% FCS (BioSell) and 100 units/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific, Waltham, USA).

Techniques: Immunofluorescence, In Vitro, Generated, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, Luminex, Derivative Assay